Whiskers denote standard error, while open rectangles for the control group and open circles for the supplemented group denote mean value. Physique 3 represents the effects of supplementation and physical exercise screening on IL-2/IL-10 in the control and supplemented groups (= 0.09, R2 = 0.05, upper CL = 0.2 lower CL = 0.02). Open in a separate window Figure 3 Effects of supplementation on IL-2/IL-10. growth factor 1 (IGF1), and WBC, lymphocyte (LYM), monocyte RAD26 (MON), and granulocyte (GRA) were measured. A statistically significant switch in IL-10 in response to the exercise program during the supplementation period in both groups was observed (= 0.01). However, the results of the rest of the comparisons were statistically insignificant ( 0.05). Contrary to our initial hypothesis, there were no significant effects of bovine supplementation around the dynamics of immune system function indicators. = 11) received four capsules of BC (produced by AGRAPAK, Poland) every morning and evening. One gel capsule contained 0.4 g of colostrum. The composition of the product per single dose of 3.2 g (four capsules): total protein-2.620 g, lactose-0.16 g, fat-0.05 g, active protein substances (lactoferrin-30 mg, Platelet-rich plasma (PRP)-0.16 g, IgG-1050 mg, IGF-16 g, LZM-21.2 mg, and LA-30 mg). The PRP content was estimated by measuring the content and ratio of amino acids (Pro and Val) based on the conducted research and analysis of bibliographic data . The placebo group (= 9) in the same dose, form, and date of the competitors received powdered milk. The composition of the placebo calculated for a single dose of 3.2 g: lactose 1.6 g, protein 1.08 g, fat 0.04 g, and ash 0.25. The supplementation period lasted for 24 weeks in total. 2.3. Physical Overall performance Examination Before the supplementation, after Relugolix 3 and 6 months, all the players performed the maximum stress test around the HP Cosmos treadmill machine. During the test, the aerobic capacity of the participants was assessed. The test Relugolix protocol was as follows: the starting speed of the treadmill machine for the runners was 8.0 km/h, then it was increased every 2 min by 1.0 km/h, until exhaustion. Participants were verbally motivated to continue as long as possible. Heart rate (bpm) was recorded with a sport tester (Polar PE 3000). The data are offered in Table 1. Table 1 Basic characteristics of the analyzed groups (mean standard deviation (SD)). = 2 underwent injury related to undergoing physical exercise program applied in a sports team, = 1 resigned without giving a specific reason). Four subjects in total resigned in the placebo group (= 1 due to the injury related to undergoing physical exercise program applied in sports team, = 1 resigned due to acute contamination, = 2 resigned from participating in a competitive sport during the trial). Eventually, results from eleven participants in supplemented and nine subjects from your placebo group were analyzed (Physique 1). No important harms or unintended effects were noted in each group. Open in a separate window Physique 1 Recruitment process of participants to the trial. 3.2. Participants Examined Table 2 presents information around the supplemented and control groups before the intervention. Table 2 Basic characteristics of the analyzed groups (mean standard deviation). = 11)= 9)= 0.01, R2 = 0.09, upper CL = 0.2 lower CL = 0.03). The post-hoc analysis showed significantly higher IL-10 values after the end of the training period 3 h after exercise testing compared to those in the control group, while in the supplemented group, exercise testing did not increase IL-10 3 h after compared to that with rest before. The IL-10 level increased in time point III compared Relugolix to those in time points I and II in both groups ( 0.05). Physique 2 represents the effects of supplementation and physical exercise screening on IL-10 in the control and supplemented groups. All the results of the post-hoc analysis are outlined in Table S1..