). [PubMed] [Google Scholar] 10. class=”kwd-title” Keywords: Sorcin\Localization, Calcium\binding protein (22 kD), Adriamycin\resistant K562 5Abbreviations used in this paper:Mabmonoclonal antibodyK562/ADMadriamycin\resistant K562SDS\PAGEsodium dodecyl sulfate\polyacrylamide gel electrophoresisFCSfetal calf serumPBSphosphate\buffered salineBSAbovine serum albuminPLPperiodate\lysine\paraformaldehydekDkilodaltonERendoplasmic reticulum Referrals 1. ) Tsuruo , T. , Iida\Saito , H. , Tirofiban Hydrochloride Hydrate Kawabata , H. , Oh\hara , T. , Hamada , H. and Utakoji , T.Characteristics of resistance to adriamycin in human being myelogenous leukemia K562 resistant to adriamycin and in isolated clones . Jpn. J. PCDH9 Malignancy Res. , 77 , 682 C 692 ( 1986. ). [PubMed] [Google Scholar] 2. ) Hamada , H. and Tsuruo , T.Practical role for the 170\to 180\kDa glycoprotein specific to drug\resistant tumor cells as revealed by monoclonal antibodies . Proc, Natl. Acad. Sci. USA , 83 , 7785 C 7789 ( 1987. ). [PMC free article] [PubMed] [Google Scholar] 3. ) Pastan , I. and Gottesmann , M.Multiple\drug resistance in human tumor . N. Engl. J. Med. , 316 , 1388 C 1393 ( 1987. ). [PubMed] [Google Scholar] 4. ) Sugawara , I. , Kataoka , I. , Morishita , Y. , Hamada , H. , Tsuruo , T. , Itoyama , S. and Mori , S.Cells distribution of P\glycoprotein encoded by a multiple drug\resistant gene as revealed by a monoclonal antibody, MRK 16 . Malignancy Res. , 48 , 1926 C 1929 ( 1988. ). [PubMed] [Google Scholar] 5. ) Sugawara , I. , Nakahara , M. , Hamada , H. , Tsuruo , T. and Mori , S.Apparent stronger expression in the human being adrenal cortex than in the human being adrenal medulla of Mr 170,000C180,000 P\glycoprotein . Malignancy Res. , 48 , 4611 C 4614 ( 1988. ). [PubMed] [Google Scholar] 6. ) Koch , G. , Smith , M. , Twentyman , P. and Wright , K.Recognition of a novel calcium\binding protein (cp22) in multidrug\resistant murine and hamster cells . FEBS Lett. , 195 , 275 C 279 ( 1986. ). [PubMed] [Google Scholar] 7. ) Vehicle der Bliek , A. M. , Meyers , M. B. , Biedler , J. L. , Hes , E. and Borst , P. A.22\kd protein (sorcin/ V19) encoded by an amplified gene in multidrug\resistant cells is definitely homologous to the calcium\binding light chain of calpain . EMBO J. , 5 , 3201 C 3208 ( 1986. ). [PMC free article] [PubMed] [Google Scholar] 8. ) Hamada , H. , Ohkochi , E. , Oh\hara , T. and Tsuruo , T.Purification of the Mr 22,000 calcium\binding protein (sorcin) associated with multidrug resistance and its detection with monoclonal antibodies . Malignancy Res. , 48 , 3173 C 3178 ( 1988. ). [PubMed] [Google Scholar] 9. ) Laemmli , U. K.Cleavage of structural proteins during the Tirofiban Hydrochloride Hydrate assembly of the head of bacteriophage T4 . Nature , 227 , 680 C 685 ( 1970. ). [PubMed] [Google Scholar] 10. ) Smith , Tirofiban Hydrochloride Hydrate P. K. , Krohn , R. I. , Hermanson , G. T. , Mallia , A. K. , Gartner , F. H. , Provenzano , M. D. , Fujimoto , E. K. , Goeke , N. M. , Olson , B. J. and Klenk , D. C.Measurement of protein using bicinchoninic acid . Anal. Biochem. , 150 , 76 C 85 ( 1985. ). [PubMed] [Google Scholar] 11. ) Hsu , S. M. , Raine , L. and Fanger , H.Use of avidin\biotinperoxidase complex (ABC) in immunoperoxidase techniques . J. Histochem. Cytochem. , 29 , 577 C 580 ( 1981. ). [PubMed] [Google Scholar] 12. ) Schindler , M. , Allen , M. L. , Olinger , M. R. and Holland , J. F.Automated analysis and survival selection of anchorage\dependent cells less than normal growth conditions . Cytometry , 6 , 368 C 374 ( 1985. ). [PubMed] [Google Scholar] 13. ) Moutsatsos , I. , Wade , M. , Schindler , M. and Wang , J. L.Endogenous lectins from cultured cells: nuclear localization of carbohydrate\binding protein 35 in proliferating 3T3 fibroblasts . Proc. Natl. Acad. Sci. USA , 84 , 6452 C 6456 ( 1987. ). [PMC free article] [PubMed] [Google Scholar] 14. ) McLean , I. W. and Nakane , P. K.Periodate\lysine\em virtude de\formaldehyde fixative: a new fixative for immunoelectron microscopy . J. Histochem. Cytochem. , 22 , 1077 C 1083 ( 1974. ). [PubMed] [Google Scholar] 15. ) Tougard , C. and Picart , R.Use of pre\embedding ultra\structural.
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