Background: Currently you will find no effective vaccines against leishmaniasis and treatment using pentavalent antimonial medicines is occasionally effective and often toxic for individuals. protein (PTR1 enzyme) was then purified and assayed. Results: gene was successfully amplified and cloned into manifestation vector. Recombinant protein (PTR1 enzyme) was purified using affinity chromatography and confirmed by Western-blot and dot blot using anti-PTR1 antibody and anti-T7 tag monoclonal antibody respectively. The enzymatic assay was confirmed as PTR1 witch performed using 6-biopterin like a substrate and nicotinamide adenine dinucleotide phosphate like a coenzyme. Summary: Iranian lizard was indicated and enzymatic assay was performed successfully. parasites infect I-CBP112 millions of people worldwide . No effective vaccine is definitely available and treatment by pentavalent antimonial medicines is only occasionally effective and often toxic for individuals . Furthermore Hadighi  reported unresponsiveness to glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant parasites. Although antifolate medicines PCPTP1 are used in the treatment of other parasitic diseases like malaria they have no effect on leishmaniasis  because of the presence of the gene (parasite . Purines and pyrimidines perform many vital functions in cells. parasites lack the metabolic machinery to prepare purine nucleotides and rely on their hosts for preformed purines. This mechanism of purine salvage can be used like a potential target for anti-parasitic medicines. Because the pyrimidine biosynthetic pathways of and its host we.e. human being are similar it is thought that restorative manipulation of pyrimidine rate of metabolism in would be less effective I-CBP112 as compared to manipulation of the purine salvage pathway [6-8]. Owing to its purine salvage dependency requires an exogenous source of pteridines. In the absence of pteridines uses a salvage pathway in which the enzyme I-CBP112 PTR1 reduces pteridines such as biopterin and folate [9-11] therefore reducing the effectiveness of methotrexate–a dihydrofolate reductase (DHFR) inhibitor–in therapy [examined in 2 8 The level of sensitivity of PTR1 to the inhibitory activity of methotrexate is definitely 2000-folds less than that I-CBP112 of DHFR-thymidylate synthase . In I-CBP112 1964 Adler  reported nine varieties of lizard offers individual characteristics. In 1966 Hoar and Wallace  suggested that lizard promastigotes are observed in NNN (Novy-MacNeal-Nicolle) medium or insect vectors whereas amastigotes are observed in mammalian hosts. However we have isolated a lizard promastigote  which differs from lizard isolated previously in other countries because that lived in heart blood. This lizard was isolated using heart blood tradition . In 1990 the WHO Specialists Committee has classified lizard as belonging to the genus but others believe that lizard belongs to the trypanosome genus . Gomes-Eichelmann  reported some variations between lizard and mammalian with regard to kinetoplast nucleic acid sequences chromosomes and membrane lipids which are not the same as those reported in mammalian from Iranian lizard and characterized the resultant recombinant PTR1 enzyme by carrying out an enzymatic assay. This model can be utilized for further investigations into drug resistance and chemotherapy. MATERIALS AND METHODS I-CBP112 extraction. genes  PCR was performed using genomic DNA. Iranian lizard . Promastigote DNA was extracted as previously explained . Briefly promastigotes were cultivated in NNN medium and mass cultured in RPMI-1640 medium enriched with 10% fetal bovine serum. promastigotes were harvested by centrifugation at 12 0 ×g and washed three times with phosphate-buffered saline. Washed promastigote were lysed with lyses buffer (320 mM glucose 10 mM Tris foundation pH 8 5 mM MgCl2 2 Triton-X 100) at 37°C for 3 h and boiled for 10 min. Samples were centrifuged at 12 0 ×g for 10 min and the supernatant was transferred to a new microfuge tube where it was subjected to DNA extraction using phenol-chloroform and precipitated with ethanol. DNA polymerase (Cinnagen Iran) in a final volume of 50μl. PCR was carried out within 30 cycles: denaturation at 94°C for 30 s annealing at 65°C for 30 s and elongation at 72°C for 40 s . The PCR product was subjected to electrophoresis on 1%.