The effect of the xanthine oxidase inhibitor, 1\acetoxychavicol acetate (ACA), on 4\nitroquinoline 1\oxide (4\NQO)\induced oral carcinogenesis was investigated in male F344 rats. termination from the test (32 weeks), the incidences of tongue neoplasms and preneoplastic lesions, polyamine amounts in the tongue tissues, and cell proliferation activity approximated with regards to 5\bromodeoxyuridine (BrdU)\labeling index and by morphometric evaluation of sterling silver\stained nucleolar organizer locations protein (AgNORs) had been likened among the groupings. Nourishing of ACA at both dosages during initiation or postinitiation considerably decreased the introduction of tongue carcinoma (93C100% decrease, P 0.001) and preneoplasia (43C50% decrease for hyperplasia and 34C48% decrease for dysplasia, P 0.05). There have been no such lesions in rats given ACA by itself or those in the neglected control group. The amount of AgNORs per cell nucleus was considerably decreased by nourishing of ACA at a higher dosage (500 ppm) (29% inhibition, P 0.05). The BrdU\labeling index was also decreased by nutritional administration of ACA (23C32% inhibition, P 0.01). Furthermore, ACA feeding decreased tongue polyamine amounts (35C40% inhibition, P 0.05). These outcomes indicate that ACA inhibited rat dental carcinogenesis, and such inhibition may be linked to suppression of cell proliferation in the dental mucosa with the xanthine oxidase inhibitor. Crazy. and their anti\ulcer actions . Chem. KOS953 Pharm. Bull. , 24 , 2377 C 2382 ( 1976. ). [PubMed] 25. ) Noro T. , Sekiya T. , Katoh M. , Oda Y. , Miyase T. , Kuroyanagi M. , Ueno A. and Fukushima S.Inhibitors of xanthine oxidase from em Alpinia galanga /em ? Chem. Pharm. Bull. , 36 , 244 C 248 ( 1988. ). 26. ) Pence B. C. and Reiners J. J. J.Murine epidermal xanthine oxidase activity: correlation with amount of hyperplasia induced by tumor promoters . Cancers Res. , 47 , 6388 C 6392 ( 1987. ). [PubMed] 27. ) Kondo A. , Ohigashi H. , Murakami A. , Suratwadee J. and Koshimizu K.1\Acetoxychavicol acetate being a potent inhibitor of tumor promoter\induced Epstein\Barr pathogen activation from em Languas galanga /em , a normal Thai condiment . Biosci. Biotechnol. Biochem. , 57 KOS953 , 1344 C 1345 ( 1993. ). 28. ) Kokoglu E. , Belce A. , Ozyurt E. and Tepeler Z.Xanthine oxidase amounts in mind tumors . Cancers Lett. , 50 , 179 C 181 ( 1990. ). [PubMed] 29. ) Reiners J. J. J. , Thai G. , Rupp T. and Cantu A. R.Evaluation from the antioxidant/prooxidant position of murine epidermis following localized treatment with 12\ em O /em \tetradecanoyl\phorbol\13\acetate and through KOS953 the entire ontogeny of epidermis cancer. Component I. Quantitation of superoxide dismutase, catalase, glutathione peroxidase and xanthine oxidase . Carcinogenesis , 12 , 2337 C 2343 ( 1991. ). [PubMed] 30. ) Tanaka T. , Makita H. , Ohnishi M. , Hirose Y. , Wang A. , Mori H. , Satoh K. , Hara A. and Ogawa H.Chemoprevention of 4\nitroquinoline 1\oxide\induced mouth carcinogenesis by eating curcumin and hesperidin: evaluation using the protective aftereffect of \carotene . Cancers Res. , 54 , 4653 C 4659 ( 1994. ). [PubMed] 31. ) Deschner E. E. , Ruperto J. , Wong G. and Newmark H. L.Quercetin and rutin seeing that inhibitors of azoxymethane induced colonic neoplasia . Carcinogenesis , 12 , 1193 C 1196 ( 1991. ). [PubMed] 32. ) Nunoshiba T. and Demple B.Powerful intracellular oxidative stress Rabbit Polyclonal to VAV1 (phospho-Tyr174) exerted with the carcinogen 4\nitroquinoline\ em N /em \oxide . Cancers Res. , 53 , 3250 C 3252 ( 1993. ). [PubMed] 33. ) Murakami A. , Nakamura Y. , Koshimizu K. and Ohigashi H.Research on the systems of 1\acetoxychavicol acetate underlying it is cancer preventive impact . Proc. Jpn. Cancers Assoc, 54th Annu. Match. , 139 ( 1995. ). 34. ) Bnczy J. and Csiba A.Incident of epithelial dysplasia in mouth leukoplakia ..